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We then pre-incubated early ring-stage artemisinin resistant and sensitive parasites with BSO for 1 h prior to pulse treatment with 700 nM of DHA for 6 h and measured survival, analogous to the standard in vitro Ring-stage Survival Assay (RSA) for artemisinin resistance

The absorbance was promptly recorded at a wavelength of 340 nm utilizing a microplate reader and compared against the absorbance of the blank solution 37

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This is the membership and its what makes the care continuous